An overview on current molecular tools for heterologous gene expression in Trichoderma

Table 1 Commonly used selection markers in Trichoderma spp.

Gene	Selection principle	Type	Species	Refs.
pyr4	Uridine prototrophy, 5 ‘-FOA sensitivity	Aux	Trichoderma reesei	[59, 106]
pyr2	Uridine prototrophy, 5 ‘-FOA sensitivity	Aux	Trichoderma reesei	[4]
asl1	Arginine prototrophy	Aux	Trichoderma reesei	[59]
ade2	Adenine prototrophy	Aux	Trichoderma reesei	[4]
his1	Histidine prototrophy	Aux	Trichoderma reesei	[32]
ben	Benomyl resistance	Het	Neurospora crassa	[107]
ble	Bleomycin/phleomycin resistance	Het	Streptoalloteichus hindustanus	[108]
hphB	Hygromycin resistance	Het	Escherichia coli	[109]
ptrA	Pyrithiamine resistance	Het	Aspergillus oryzae	[110]
nptII	Geneticin/neomycin resistance	Het	Escherichia coli	[111]
amdS	Ability to use acetamide as N-source, fluoroacetamide sensitivity	Het	Aspergillus nidulans	[6]

ISSN: 2054-3085