Fig. 8
From: An overview on current molecular tools for heterologous gene expression in Trichoderma
Usage of a 2A peptide in a bicistronic gene construct yielding two independent proteins. The coding regions for eGFP and the cellulase Cel7a are transcribed together using the same promoter (P) and terminator (T). The coding regions are spanned by the genetic sequence for a 2A peptide. During the translation, the 2A peptide leads to a “skipping” of the ribosome; it fails to make a peptide bond between the C-terminal proline and the previous amino acid in the 2A peptide