Fig. 5
From: An overview on current molecular tools for heterologous gene expression in Trichoderma
Marker recycling using the Cre/loxP system. A The selection marker (SM) is flanked by two direct repeats of a LoxP site. Recombination of the two LoxP sites by the Cre recombinase leads to excision of the selection marker. In this system, the gene encoding for the Cre recombinase is located in a different locus. B In the LML 3.0 system, the cre gene is part of the deletion cassette. A light inducible activator (A) induces expression of Cre, which leads to excision of the cre gene together with the SM. P, promoter; T, terminator