Fig. 4

Fluorescence microscopy images of S. cerevisiae strains expressing mitochondrially targeted mRuby2 together with different Ura9 orthologs fused to eGFP. Cells were grown on SMUD for at least two duplications and fluorescence of eGFP, mRuby and MitoTracker Deep Rep FM was detected by fluorescence microscopy. For each strain, from top to bottom: mRuby2/MitoTracker Deep Red FM fluorescence specifically localized to mitochondria, indicating localization of mitochondrial mass; eGFP fluorescence, tagged to different Ura9 orthologs, indicating subcellular Ura9 localization; phase-contrast image; and an overlay of all channels. From left to right; A IME604, expressing OpURA9-eGFP, B IME601 (DbURA9-eGFP), C IME600 (Arura9-eGFP), D IME602, (SjURA9-eGFP) and E IME602 stained with MitoTracker Deep Red FM. Scale bars are equivalent to 10 μm. Pictures are a representation of the full culture