Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing

Table 1 Gene editing efficiencies of P. variotii and P. roqueforti using CRISPR/Cas9

	P. variotii		P. roqueforti
	Brown conidia	White conidia	Green conidia	White conidia
Phenotype obtained on first transformation plate	148/876 (17%)	728/876 (83%)	2/58 (3%)	56/58 (97%)
Hygromycin resistance loss after one round of non-selective growth	32/40 (80%)	9/40 (23%)	1/2 (50%)	16/56 (29%)

Numbers represent transformants and were calculated over multiple transformation experiments. The average amount of transformants obtained per transformation using 2 µg of CRISPR/Cas9 plasmid was ± 100 colonies for P. variotii and ± 10 colonies for P. roqueforti depending on amounts of obtained protoplasts

ISSN: 2054-3085