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Table 1 Gene editing efficiencies of P. variotii and P. roqueforti using CRISPR/Cas9

From: Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing

  P. variotii P. roqueforti
  Brown conidia White conidia Green conidia White conidia
Phenotype obtained on first transformation plate 148/876 (17%) 728/876 (83%) 2/58 (3%) 56/58 (97%)
Hygromycin resistance loss after one round of non-selective growth 32/40 (80%) 9/40 (23%) 1/2 (50%) 16/56 (29%)
  1. Numbers represent transformants and were calculated over multiple transformation experiments. The average amount of transformants obtained per transformation using 2 µg of CRISPR/Cas9 plasmid was ± 100 colonies for P. variotii and ± 10 colonies for P. roqueforti depending on amounts of obtained protoplasts