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Fig. 3 | Fungal Biology and Biotechnology

Fig. 3

From: Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing

Fig. 3

Heat resistance of three food spoiling fungi and their melanin deficient mutants. Colours used correspond with the phenotype of the conidia, see Fig. 2a The heat inactivation curves of A. niger N402 wild-type conidia (black lines) and A. niger MA93.1 melanin deficient mutant conidia (brown lines). The A. niger strains were heat treated in a 56 °C water bath. Heat inactivation shows only a 2-log reduction in microbial load for wild-type and mutant when treated for 30 min. b The heat inactivation curves of P. variotii CBS101075 wild-type conidia (light brown lines) and P. variotii PT32.5 melanin deficient mutant conidia (grey lines). The P. variotii strains were heat treated in a 60 °C water bath. Heat inactivation shows a 3-log reduction in microbial load for wild-type and mutant when treated for 30 min. c The heat inactivation curves of P. roqueforti LCP9604111 wild-type conidia (green lines) and P. roqueforti PT34.2 melanin deficient mutant conidia (grey lines). The P. roqueforti strains were heat treated in a 56 °C water bath. Heat inactivation shows at least a 5-log reduction in microbial load for wild-type and mutant when treated for 30 min. Three biological triplicates were measured. Inactivation curves were drawn based on the linear regression model

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