Fig. 1

Transformation plates obtained when transforming P. variotii and P. roqueforti with PKS gene targeting CRISPR/Cas9 plasmids. a Transformation plate of P. variotii CBS101075 transformed with AMA1-based CRISPR/Cas9 plasmid pPT13.1 targeting gene pvpP needed for melanin biosynthesis. Typically around 100 transformants are obtained when adding 2 µg of plasmid depending on the amount of protoplasts obtained. b Transformation plate of P. roqueforti LCP9604111 transformed with AMA1-based CRISPR/Cas9 plasmid pPT9.3 targeting gene pksA. Typically around 10 transformants are obtained when adding 2 µg of plasmid depending on the amount of protoplasts obtained