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Fig. 1 | Fungal Biology and Biotechnology

Fig. 1

From: Optimization of scleroglucan production by Sclerotium rolfsii by lowering pH during fermentation via oxalate metabolic pathway manipulation using CRISPR/Cas9

Fig. 1

Selection and identification of the mutants by using hygromycin-BPDA medium and sequencing. A Sequences of single colonies detected after selection of the hygromycin-BPDA top agar. Deletions are marked by dashes. Insertions and PAMs are marked in blue and purple, respectively. Numbers to the right of the sequences indicate the net loss or gain of bases for each sequence, with the number of bases inserted ( +) or deleted ( −) indicated in parentheses. B A rapid method to select mutants compared with the control based on the medium color. Protoplasts of WT cultured in hygromycin-BPDA medium after 5 days (a) and 8 days (b), respectively. Protoplasts of S. rolfsii operated by PEG-mediated transformation of RNPs cultured in hygromycin-BPDA medium after 5 days (c) 8 days (d), respectively

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