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ade8_f
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GAATTGGGTACCGGGCCCCCCCTCGAGGTCGACTGGCCGTTCATAGCGATGTC (sequence upstream of the HindIII-site in pRS426 in italic, sequence upstream of ade8+ in normal letters)
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Cloning of ade8+ in pCcAde8
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ade8_r
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GCCGCTCTAGAACTAGTGGATCCCCCGGGCTGAGCTCGTTTCCATCGTCATCA (sequence downstream of the EcoRI-site in pRS426 in italic, sequence downstream of ade8+ in normal letters)
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Cloning of ade8+ in pCcAde8
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Lcc5-fwd
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CTCCCATCTACACACAACAAGCTTATCGCCATGTCGTTTGCTTGGAAAGCATTGGC (A. bisporus Pgpd sequence is in italic, lcc5 sequence in normal letters)
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Cloning of lcc5 for overexpression in pYSK-lcc5
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Lcc5-rev
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CCACTGGCCCTCTGGTCAACTATAATATTATTTAGGGATACATAGGGAGCAAGTTCGAA (Tlcc1 sequence is in italic, lcc5 sequence in normal letters)
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Cloning of lcc5 for overexpression in pYSK-lcc5
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Lcc9-fwd
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CTCCCATCTACACACAACAAGCTTATCGCCATGTCCAGGAAACTTTTCTCTCTCGCC (A. bisporus Pgpd sequence is in italic, lcc9 sequence in normal letters)
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Cloning of lcc9 for overexpression in pYSK-lcc9
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Lcc9-rev
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CCACTGGCCCTCTGGTCAACTATAATATTATTTAAGGAGTGGGGACAATTTGGATAGAGGT (Tlcc1 sequence is in italic, lcc9 sequence in normal letters)
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Cloning of lcc9 for overexpression in pYSK-lcc9
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Lcc9-antisense 1-fwd
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CTCCCATCTACACACAACAAGCTTATCGCCCGGGATTCTCATAGTTGTAAGTGCTGC (A. bisporus Pgpd sequence is in italic, lcc9 antisense 1 sequence in normal letters)
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Cloning of lcc9-antisense fragment 1 in pYSK-lcc9-antisense-1
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Lcc9- antisense 1-rev
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CACTGGCCCTCTGGTCAACTATAATATTATAGATGGGCCTTGGACCTGCCG (Tlcc1 sequence is in italic, lcc9 antisense 1 sequence in normal letters)
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Cloning of lcc9-antisense fragment 1 in pYSK-lcc9-antisense-1
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Lcc9- antisense 2-fwd
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CTCCCATCTACACACAACAAGCTTATCGCCCGGACCACTTCCTCCTGGGGCA (A. bisporus Pgpd sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of lcc9-antisense fragment 2 in pYSK-lcc9-antisense-2
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Lcc9- antisense 2-rev
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CACTGGCCCTCTGGTCAACTATAATATTATCTCTCATGGTCGACGAAATCCAGATC (Tlcc1 sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of lcc9-antisense fragment 2 in pYSK-lcc9-antisense-2
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Pgpd-F
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GATATCGAAGAAGAATTCAGAGGTCCGCAAGTA (A. bisporus Pgpd sequence, EcoRV site underlined)
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Cloning of A. bisporus gpdII promotor for pCRII-hph-lcc9 vector construction
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Pgpd-R
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AAGTGGTCCGGGCGATAAGCTTGTTGTGTGTAGATGG (A. bisporus Pgpd sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of A. bisporus gpdII promotor for pCRII-hph-lcc9 vector construction
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Lcc9-antisense-hphF
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GCTTATCGCCCGGACCACTTCCTCCTGGGGCA (A. bisporus Pgpd sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of lcc9 antisense fragment 2 for pCRII-hph-lcc9 vector construction
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Lcc9-antisense-hphR
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TGCTATGACTCTCTCATGGTCGACGAAATCCAGATC (Tlcc9 sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of lcc9 antisense fragment 2 for pCRII-hph-lcc9 vector construction
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Tlcc9-F
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ACCATGAGAGAGTCATAGCACATAGCCATACCGACAC (Tlcc9 sequence is in italic, lcc9 antisense 2 sequence in normal letters)
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Cloning of C. cinerea lcc9 terminator for pCRII-hph-lcc9 vector construction
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Tlcc9-R
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GGGCCCGTCAAAGGAGTCAGCCCTTGGACATG (Tlcc9 sequence, ApaI site underlined)
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Cloning of C. cinerea lcc9 terminator for pCRII-hph-lcc9 vector construction
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DPf
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ATGTCGATCCGCATCCTACTCCTC (sequence of ade8 from startcodon onwards)
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Diagnosis PCR for nuclear ade8+ insertion
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DPr
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ATCCCAGGCGGAGAGATTGCG (sequence of ade8 with its last triplets for amino acids)
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Diagnosis PCR for nuclear ade8+ insertion
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PF
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ACATCCACCATCTCCGTTTTCTCCCAT (A. bisporus Pgpd sequence)
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PCR of OK130 co-transformants of lcc9-antisense-constructs
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PR
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TGACTATAGCAGCCTCCTACCACTG (Tlcc1 sequence)
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PCR of OK130 co-transformants of lcc9-antisense-constructs
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qRT-lcc9-F
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ATGTCCAGGAAACTTTTCTCTCTCG (lcc9 sequence + 1 to + 25)
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qRT-PCR of lcc9
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qRT-lcc9-R
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ATGTTCGAGACCGTCATGGTACT (reverse complementary lcc9 sequence of + 79 to + 101)
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qRT-PCR of lcc9
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