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Table 4 Transformations of C. cinerea PG78 (trp1.1,1.6, pab1-1) with either trp1+ plasmid pBD5 or pab1+ vector pPAB1-2 alone or, using same batches of protoplasts, in combination with other non-directly selectable vectors

From: Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of Coprinopsis cinerea

Plasmid(s) Transformants collected on Total transformants Ratio of clones
1st day 2nd day 3rd day 4th day 5th day 6th day 7th day
Experiment 1: trp1+ selection
pBD5 21 26 14 6 67 1.0
pBD5 + pYSK7 10 16 31 31 14 4 0 106 1.6
pBD5 + pDB3 2 4 0 50 69 15 12 152 2.3
pBD5 + pCcAde8 45 67 20 16 148 2.2
pab1+ selection
pPAB1-2 40 18 14 8 80 1.0
pPAB1-2 + pYSK7 40 13 40 11 - 104 1.3
pPAB1-2 + pDB3 53 11 31 8 4 107 1.3
pPAB1-2 + pBD5 14 6 19 13 15 3 70 0.9
pPAB1-2 + pCcAde8 59 32 49 9 3 152 1.9
Experiment 2: trp1+ selection
pBD5 20 21 20 15 7 83 1.0
pBD5 + pYSK7 26 42 31 13 13 125 1.5
pBD5 + pDB3 34 38 29 13 7 121 1.5
pBD5 + pCcAde8 18 27 49 16 12 122 1.5
pab1+ selection
pPAB1-2 25 29 50 19 13   136 1
pPAB1-2 + pYSK7 40 19 37 37 12 145 1.1
pPAB1-2 + pDB3 33 46 33 26 17 155 1.1
pPAB1-2 + pBD5 7 30 37 25 18 117 0.9
pPAB1-2 + pCcAde8 37 32 54 38 18 177 1.3
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