Table 4 Transformations of C. cinerea PG78 (trp1.1,1.6, pab1-1) with either trp1+ plasmid pBD5 or pab1+ vector pPAB1-2 alone or, using same batches of protoplasts, in combination with other non-directly selectable vectors
Plasmid(s) | Transformants collected on | Total transformants | Ratio of clones | ||||||
|---|---|---|---|---|---|---|---|---|---|
1st day | 2nd day | 3rd day | 4th day | 5th day | 6th day | 7th day | |||
Experiment 1: trp1+ selection | |||||||||
pBD5 | – | – | – | 21 | 26 | 14 | 6 | 67 | 1.0 |
pBD5 + pYSK7 | 10 | 16 | 31 | 31 | 14 | 4 | 0 | 106 | 1.6 |
pBD5 + pDB3 | 2 | 4 | 0 | 50 | 69 | 15 | 12 | 152 | 2.3 |
pBD5 + pCcAde8 | – | – | – | 45 | 67 | 20 | 16 | 148 | 2.2 |
pab1+ selection | |||||||||
pPAB1-2 | 40 | 18 | 14 | 8 | – | – | – | 80 | 1.0 |
pPAB1-2 + pYSK7 | 40 | 13 | 40 | 11 | – | – | - | 104 | 1.3 |
pPAB1-2 + pDB3 | 53 | 11 | 31 | 8 | 4 | – | – | 107 | 1.3 |
pPAB1-2 + pBD5 | 14 | 6 | 19 | 13 | 15 | 3 | – | 70 | 0.9 |
pPAB1-2 + pCcAde8 | 59 | 32 | 49 | 9 | 3 | – | – | 152 | 1.9 |
Experiment 2: trp1+ selection | |||||||||
pBD5 | 20 | 21 | 20 | 15 | 7 | – | – | 83 | 1.0 |
pBD5 + pYSK7 | 26 | 42 | 31 | 13 | 13 | – | – | 125 | 1.5 |
pBD5 + pDB3 | 34 | 38 | 29 | 13 | 7 | – | – | 121 | 1.5 |
pBD5 + pCcAde8 | 18 | 27 | 49 | 16 | 12 | – | – | 122 | 1.5 |
pab1+ selection | |||||||||
pPAB1-2 | 25 | 29 | 50 | 19 | 13 |  | – | 136 | 1 |
pPAB1-2 + pYSK7 | 40 | 19 | 37 | 37 | 12 | – | – | 145 | 1.1 |
pPAB1-2 + pDB3 | 33 | 46 | 33 | 26 | 17 | – | – | 155 | 1.1 |
pPAB1-2 + pBD5 | 7 | 30 | 37 | 25 | 18 | – | – | 117 | 0.9 |
pPAB1-2 + pCcAde8 | 37 | 32 | 54 | 38 | 18 | – | – | 177 | 1.3 |