Table 2 Transformations of C. cinerea OK130 (ade8-1) with ade8+-vector pCcAde8 alone or, using same batches of protoplasts, in combination with various pYSK7 laccase gene derivatives
Plasmid(s) | ade8+-selected transformants collected on* | Total transformants* | |||
|---|---|---|---|---|---|
1st day | 2nd day | 3rd day | 4th day | ||
Experiment 1: Laccase overexpression | |||||
pCcAde8 | 17 (1) | 15 | 7 | 2 | 41 |
pCcAde8 + pYSK7 | 26 (8) | 20 (13) | 7 (3) | 7 (1) | 60 (25) |
pCcAde8 + pYSK-lcc5 | 14 (2) | 27 (8) | 25 (5) | 10 (5) | 76 (20) |
pCcAde8 + pYSK-lcc9 | 10 (4) | 23 (10) | 23 (5) | 8 (0) | 64 (19) |
Experiment 2: Laccase silencing | |||||
pCcAde8 | 17 | 20 | 12 | 6 | 55 |
pCcAde8 + pYSK-lcc9-antisense-1 | 5 (2) | 7 (2) | 12 (6) | 6 (4) | 30 (14) |
pCcAde8 + pYSK-lcc9-antisense-2 | 2 (1) | 9 (5) | 17 (12) | 8 (6) | 36 (24) |