Fig. 4

Untransformed ade8-1 monokaryon OK130 (top left) and pCcAde8 transformed clones (top right) with barely detectable halos from background laccase activity on ABTS and pCcAde8 + pYSK-lcc9 transformants (bottom) with strongly stained broad halos of enzymatically oxidized ABTS. Clones were grown on regeneration agar medium which 0.5 mM ABTS and 50 mg/L adenine sulphate