Fig. 2

Neighbor-joining phylogenetic tree of bifunctional fungal GARS-AIRS enzymes clustering according to fungal clades. Note that corrections in exon/intron splicing sites have been done for the OK130 Ade8 model (GenBank EAU92737.2 = Broad model CC1G_ 01782T0 = JGI ID 1589), following the RNAseq-supported model for the ade8⁺ gene of strain AmutBmut (JGI ID 414375). The Drosophila melanogaster Ade3 protein used as outgroup is trifunctional with GARS, AIRS and GART domains, the latter of which was excluded from the analysis