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Table 1 Characteristics of proteins identified in Z. tritici EV samples and the soluble secreted fraction

From: Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Proteins identified using LC–MS/MS EV proteins Soluble secreted proteins (SS)
Biological replicates 5 3
Total proteins identified 771 99
High confidence (HC) proteins1 240 79
HC proteins unique to sample type2 210 (100%) 49 (100%)
 Proteins with signal peptides 14 (6.7%) 38 (77.6%)
 Proteins with transmembrane domains/GPI-anchors3 15 (7.1%) 1 (2.0%)
 Putative Cazymes 7 (3.3%) 6 (12.2%)
 Putative proteolytic enzymes 6 (2.5%) 3 (6.1%)
 Predicted effectors4 17 (8.1%) 16 (32.7%)
 Median protein size 426 aa 257 aa
  1. 1Proteins were high-confidence if present in ≥ 4/5 EV biological replicates or ≥ 2/3 SS replicates, with ≥ 2 unique peptides detected
  2. 2Protein counts and percentages in the following rows are in reference to the subset of HC proteins detected only in EVs or SS fractions; proteins detected in both fractions were excluded from these counts
  3. 3Transmembrane domains predictions by TMHMM were included when more than one domain predicted, or if only one was predicted, this was not in the first 60 amino acids
  4. 4Effector count includes proteins predicted as effector by both v1.0 and v2.0 of EffectorP