Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Table 1 Characteristics of proteins identified in Z. tritici EV samples and the soluble secreted fraction

Proteins identified using LC–MS/MS	EV proteins	Soluble secreted proteins (SS)
Biological replicates	5	3
Total proteins identified	771	99
High confidence (HC) proteins¹	240	79
HC proteins unique to sample type²	210 (100%)	49 (100%)
Proteins with signal peptides	14 (6.7%)	38 (77.6%)
Proteins with transmembrane domains/GPI-anchors³	15 (7.1%)	1 (2.0%)
Putative Cazymes	7 (3.3%)	6 (12.2%)
Putative proteolytic enzymes	6 (2.5%)	3 (6.1%)
Predicted effectors⁴	17 (8.1%)	16 (32.7%)
Median protein size	426 aa	257 aa

¹Proteins were high-confidence if present in ≥ 4/5 EV biological replicates or ≥ 2/3 SS replicates, with ≥ 2 unique peptides detected
²Protein counts and percentages in the following rows are in reference to the subset of HC proteins detected only in EVs or SS fractions; proteins detected in both fractions were excluded from these counts
³Transmembrane domains predictions by TMHMM were included when more than one domain predicted, or if only one was predicted, this was not in the first 60 amino acids
⁴Effector count includes proteins predicted as effector by both v1.0 and v2.0 of EffectorP

ISSN: 2054-3085