Fig. 7

Transcript levels of the main cellulases-, xylanases- and the aldose reductase encoding genes. The SynX-bearing strain TXYE was pre-cultivated on in shake flasks on glycerol without 17β-estradiol for 24 h. Acquired mycelium was filtered and washed and then equal amounts were transferred to medium containing no carbon source (NC), glucose (Glu), glycerol (Gly), 1 mM xylose (XO), or lactose (Lac) and either 300 nM 17β-estradiol (Estr.) or only the solvent DMSO as control. Samples were taken after 3 and 6 h of incubation, the total RNA was isolated and the relative transcript levels of the indicated genes were determined by a RT-qPCR assay normalized to the reference sample (indicated by an asterisk) using the reference genes sar1 and act1 for normalization. X represents no detection. Error bars indicate standard deviation from three independently grown cultures