Fig. 2

Adalimumab production in the culture supernatant of A. oryzae. The producing strains were grown in the 5 × DPY medium. The host strains used for transformation to produce adalimumab were as follows: NSlD1, control strain; NSlDv10, strain with the gene deletion of Aovps10 for the vacuolar protein sorting receptor; AUT1-lD-v10-sD, hyper-producing mutant (AUT1) strain with double deletion of the tripeptidyl peptidase gene sedD and Aovps10; NSlD-ΔP10, ten-protease genes deletion strain. Data present the mean of three independent experiments with error bars indicating SD. *, p ≤ 0.05; **, p ≤ 0.01 (t test)