Fig. 10
From: Functional production of human antibody by the filamentous fungus Aspergillus oryzae
TNFα neutralizing activity of adalimumab produced by A. oryzae. For neutralization, 20 ng/mL human TNFα and 1 µg/mL actinomycin D were mixed with different concentration of antibodies. Then, 100 µL of these mixtures were added to the well containing MDA-MB-468 cells. The cell viability was measured by MTT assay at OD 600 nm. Each bar represents the standard deviation of the mean. n = 3