Skip to main content
Fig. 3 | Fungal Biology and Biotechnology

Fig. 3

From: Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum

Fig. 3

a A schematic diagram of Tox3-HDR cassette with 50 bp microhomology arms targeting Tox3 gene. b PCR amplification of eight mutant colonies where the mutant (with hygromycin insertion) predicted band size is 2585 bp. The screening primers were designed such that a product will only amplify in the event of homologous recombination (i.e. no product will amplify in the wild-type). MH represents microhomology mutant

Back to article page