Fig. 2
From: Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum
Disruption of Tox3 gene generated using the Tox3-RNP complex and Tox3-HDR cassette with 1 kb homologous flanks to Tox3 gene. a A sequence schematic highlighting the sequence of the gRNA and location of the targeted site in the Tox3 sequence. b Design of an HDR cassette with 1 kb homologous flanks on each side. c A gel electrophoresis image showing that the Tox3-HDR cassette was intact in presence of Tox3-RNP complex. d PCR screening of 5 selected Tox3 mutants with 4.2 kb band size (predicting insertion of hygromycin cassette in the target site) and wild type with a band size of 1.5 kb. e Multiple sequence alignment of the five mutants screened above demonstrating integration into the expected target cut site