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Fig. 1 | Fungal Biology and Biotechnology

Fig. 1

From: Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum

Fig. 1

CRISPR/Cas9 genome editing of Tox3 in P. nodorum via delivery of Tox3-RNP complex. a A sequence schematic highlighting the sequence of the gRNA and location of the targeted site in the Tox3 gene. b A gel image demonstrating the in vitro cleavage of Tox3 in the presence of the Tox3-RNP complex. Bands of 1200 bp and 370 bp are consistent with the sizes expected for the targeted site within Tox3 after cleavage. c Sequence alignments of the targeted Tox3 region in selected mutants. The sequences demonstrate a variety of SNPs and small indels consistent with the activity of Cas9 at the targeted site. All strains screened were mutated implying a 100% efficiency of editing at the Tox3 locus

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