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Table 2 Expression plasmids constructed in this study

From: Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica

Plasmid nameExpression cassette (promoter-gene-terminator)Selection marker for Y. lipolytica
pLif_075pTpi1-MgHpsPhi-tTef1Hygromycin
pLif_085pFba1-CbDhas_tTdh3Hygromycin
pLif_086pFba1-CbDhas_syta-tTdh3Hygromycin
pLif_115pTpi1-BmHps-tTef1-pTdh3-BmPhi-tPgk1Hygromycin
pLif_164pScPgk1-BmHps_Sc-tScAdh1Leucine
pLif_165pScPgk1-BmHps_Sc-tScAdh1Leucine
pLif_166pFba1-BgHps-tTdh3Leucine
pLif_167pFba1-BpHps-tTdh3Leucine
pLif_168pFba1-BsHps-tTdh3Leucine
pLif_169pFba1-HmHps-tTdh3Leucine
pLif_170pFba1-LeeHps-tTdh3Leucine
pLif_171pFba1-LbHps-tTdh3Leucine
pLif_172pFba1-BgHps-tTdh3Leucine
pLif_173pFba1-MvHps-tTdh3Leucine
pLif_174pFba1-RtHps-tTdh3Leucine
pLif_175pFba1-BmHps_native-tTdh3Leucine
pLif_176pFba1-BmHps-tTdh3Leucine
  1. pLif_165 is a multicopy vector, all other plasmids are centromeric vectors
  2. aCytosolic version of CbDhas was created by omitting the three C-terminal amino acids from the protein