Table 2 Expression plasmids constructed in this study
From: Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica
Plasmid name | Expression cassette (promoter-gene-terminator) | Selection marker for Y. lipolytica |
|---|---|---|
pLif_075 | pTpi1-MgHpsPhi-tTef1 | Hygromycin |
pLif_085 | pFba1-CbDhas_tTdh3 | Hygromycin |
pLif_086 | pFba1-CbDhas_syta-tTdh3 | Hygromycin |
pLif_115 | pTpi1-BmHps-tTef1-pTdh3-BmPhi-tPgk1 | Hygromycin |
pLif_164 | pScPgk1-BmHps_Sc-tScAdh1 | Leucine |
pLif_165 | pScPgk1-BmHps_Sc-tScAdh1 | Leucine |
pLif_166 | pFba1-BgHps-tTdh3 | Leucine |
pLif_167 | pFba1-BpHps-tTdh3 | Leucine |
pLif_168 | pFba1-BsHps-tTdh3 | Leucine |
pLif_169 | pFba1-HmHps-tTdh3 | Leucine |
pLif_170 | pFba1-LeeHps-tTdh3 | Leucine |
pLif_171 | pFba1-LbHps-tTdh3 | Leucine |
pLif_172 | pFba1-BgHps-tTdh3 | Leucine |
pLif_173 | pFba1-MvHps-tTdh3 | Leucine |
pLif_174 | pFba1-RtHps-tTdh3 | Leucine |
pLif_175 | pFba1-BmHps_native-tTdh3 | Leucine |
pLif_176 | pFba1-BmHps-tTdh3 | Leucine |