Fig. 3

Construction and validation of overexpression mutant Fs OE::fsr6. a Illustration of fsr gene cluster orthologues in Fusarium solani, F. fujikuroi and F. graminearum. b Workflow for replacement of the eYFP gene for fsr6 in the agrobacterium shuttle vector pSHUT4 via yeast mediated recombinational assembly. c Photographs of F. solani wild type and fsr6 overexpression mutant. d growth diameter test on Czapek dox and yeast sucrose agar media. e genome sequencing of Fs OE::fsr6 reveals correct integration of the pSHUT4-fsr6 T-DNA cassette in the locus via homologous recombination. f HPLC–MS total ion chromatograms for metabolite extracts from F. solani wild type and the fsr6 overexpression mutant in triplicates. The compounds javanicin [M + H]⁺ = 291.0868 and bostrycoidin [M + H]⁺ = 286.0715 were observed in the mutant extracts