Identification of novel citramalate biosynthesis pathways in Aspergillus niger

Table 3 Transcriptome data of putative orthologs and paralogs in A. niger to functionally characterized organic acid transporters in filamentous fungi

New locus tag	Old locus tag	Transporter	Protein sequence coverage (%)	Protein sequence similarity (%)	RPKM values
New locus tag	Old locus tag	Transporter	Protein sequence coverage (%)	Protein sequence similarity (%)	AB1.13	AB1.13 CAD	AB1.13 #49B	CitB#99
		Citrate transporter CexA A. niger (Steiger et al. [23])
ANI_1_478154	An17g01710	Citrate transport protein (cexA)	100	100	57.83	44.83	189.54	58.61
		Citramalate transporter MfsB *A. niger* (This study)
ANI_1_930084	An09g00190	MFS multidrug transporter (mfsB)	100	100	197.34	140.59	62.55	10.52
ANI_1_1618104	An12g03020	MFS multidrug transporter	96	62	0.99	1.05	2.50	8.32
		Itaconate transporter MfsA *A. terreus* (Li et al. Hossain et al. [8, 16])
ANI_1_2702024	An02g07580	MFS transporter	84	36	2.81	2.52	2.07	2.42
		Hydroxyparaconate transporter Itp1 *Ustilago maydis* (Geiser et al. Hosseinpour et al. [21, 22])
ANI_1_478154	An17g01710	Citrate transport protein (cexA)	93	37	57.83	44.83	189.54	58.61
		Malate transporter MaeA *A. oryzae* (Knuf et al. [20])
ANI_1_2040144	An16g08330	C4-dicarboxylate transporter/malic acid transport protein	97	71	1.32	1.95	1.75	1.54

RPKM values, taken from Hossain et al. [10] were calculated according to the method presented by Mortazavi et al. [28] in order to normalize data for gene length. Protein similarity and coverage scores were obtained using the BLAST algorithm (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)

ISSN: 2054-3085