Fig. 8
Plotting protein and citric acid/oxaloacetate titres reveals a possible correlation between these processes amongst A. niger GEF and GAP conditional expression mutants. Total extracellular protein (mg/g biomass) at various Dox concentrations in progenitor control (MA70.15) and all conditional expression mutants TC4.4, TC4.5, TC5.5, TC5.6 and TC6.1 were plotted as a function citric acid or oxaloacetic acid concentrations in culture supernatant (g/g depleted glucose). Trend lines were estimated for citric acid/protein titres using polynomial of degree 2 (a) or linear fit for oxaloacetic acid/protein titres (b). R2 values for these trend lines were 0.44 and 0.27, respectively