Fig. 4

Representative images and quantitative analysis of conditional expression mutant macromorphology during submerged growth in minimal media. To model protein fermentation conditions, 1 × 10⁶ spores/ml of conditional expression mutants and progenitor control (MA70.15) were inoculated in 20 ml MM with 5% glucose as carbon source and supplemented with various concentrations of Dox. Cultures were grown at 220 RPM, 30 °C, for 72 h. a Representative images are depicted for triplicated experiments each consisting of duplicate replicates. Pelleted morphologies (any fungal structure > 500 µm² area) are reported as a function of the total fungal area measured during image analysis, and are indicated as a percentage in parenthesis. Scale bar in the top left panel is 1 mm. b Shake flask cultures were quantitatively analysed using the MPD image analysis pipeline [37]. Reported are box whisker plots for pellet morphology number (MN). Crosses depict average values. Pairwise Student‘s t-tests were conducted between conditional expression mutant relative to the MA70.15 control at respective Dox concentrations. p values are indicated as (< 0.05, *)