Fig. 2

Quantitative analysis of early hyphal growth in conditional expression mutants following titration of gene expression. 1 × 10⁴ spores/ml were inoculated in 10 µl volumes onto MM supplemented with various concentrations of Dox (μg/ml). Plates were incubated at 30 °C in the dark for 18 h. Box whisker plots depicting hyphal length (µm, A) and branching rate (length µm/total number of branches, B) are given. Experiments were technically triplicated. Note that secG (TC4.5) and ageB (TC5.6) mutants performed comparably to their isogenic comparator, and are omitted from this figure for clarity. Asterisk indicate significant differences between progenitor control (MA70.15) and conditional expression isolates using a Student‘s t test. A minimum of 30 hyphae were analysed per strain/condition