Skip to main content

Table 4 Editing efficiency for four different gene loci of FnCpf1

From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

TargetRNPPlasmid
Mean [%]SD [%]nNo. of analysed transformantsMean [%]SD [%]nNo. of analysed transformants
alp193.99.47108807.1478
pks4.25034.313228100010158
snc157.217.7111854010.46118
ptf176.63285.57.8238
  1. SD standard deviation, n number of transformations performed
  2. Diagnostic PCR was done for alp1 and ptf1 loci as described in “Methods” section. For snc1, fluorescence microscopy and for pks4.2, the colour of conidia was used to verify successful integration of the donor DNA. The donor DNA for pks4.2 was carrying the amdS selection marker