Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Table 4 Editing efficiency for four different gene loci of FnCpf1

Target	RNP				Plasmid
Target	Mean [%]	SD [%]	n	No. of analysed transformants	Mean [%]	SD [%]	n	No. of analysed transformants
alp1	93.9	9.4	7	108	80	7.1	4	78
pks4.2	50	34.3	13	228	100	0	10	158
snc1	57.2	17.7	11	185	40	10.4	6	118
ptf1	7	6.6	3	28	5.5	7.8	2	38

SD standard deviation, n number of transformations performed
Diagnostic PCR was done for alp1 and ptf1 loci as described in “Methods” section. For snc1, fluorescence microscopy and for pks4.2, the colour of conidia was used to verify successful integration of the donor DNA. The donor DNA for pks4.2 was carrying the amdS selection marker

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