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Table 3 Transformants and PCR-confirmed editing efficiency for the simultaneously targeted alp1, pks4.2, snc1, and ptf1 gene loci

From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

NucleaseNo. of coloniesEditing efficiency (%)
alp1pks4.2snc1ptf1
1010000
FnCpf1569136526
AsCpf1809210490
SpCas9402097020
  1. Diagnostic PCR for all individual loci were performed as described in “Methods” section