Table 3 Transformants and PCR-confirmed editing efficiency for the simultaneously targeted alp1, pks4.2, snc1, and ptf1 gene loci
From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi
Nuclease | No. of colonies | Editing efficiency (%) | |||
|---|---|---|---|---|---|
alp1 | pks4.2 | snc1 | ptf1 | ||
– | 1 | 0 | 100 | 0 | 0 |
FnCpf1 | 56 | 91 | 36 | 52 | 6 |
AsCpf1 | 80 | 92 | 10 | 49 | 0 |
SpCas9 | 40 | 20 | 97 | 0 | 20 |