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Table 2 Transformants and gene deletion efficiency targeting the pks4.2 gene

From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Nuclease

No. of colonies analysed

Editing efficiency (%)

9

25

FnCpf1

43

100

AsCpf1

15

100

SpCas9

11

100

  1. Phenotypic screens and diagnostic PCR for the pks4.2 locus were performed as described in “Methods” section