Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Table 1 Filamentous fungal model strains and cell factories for which CRISPR gene editing tools have been established.

Species	CRISPR protein	Nuclease delivery	References
Aspergillus fumigatus	Cas9	Plasmid-based and RNP-based	[36, 37]
A. nidulans	Cas9, Cpf1	Plasmid-based	[9]
A. niger	Cas9, Cpf1	Plasmid-based and RNP-based	[5, 9]
A. oryzae	Cas9	Plasmid-based	[38]
Neurospora crassa	Cas9	Plasmid-based	[39]
Penicillium chrysogenum	Cas9	Plasmid- based and RNP-based	[33]
Thermothelomyces thermophilus	Cas9	Plasmid-based	[12]
Trichoderma reesei	Cas9	Plasmid-based	[40]

Note that the nuclease can be delivered to the cell either plasmid encoded or as a purified protein, which has to be preassembled with in vitro transcribed gRNA to form ribonucleoproteins (RNPs)

ISSN: 2054-3085