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Fig. 1 | Fungal Biology and Biotechnology

Fig. 1

From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Fig. 1

Phenotypes of T. thermophilus strains generated with CRISPR. a Deletion-phenotype of pks4.1 and pks4.2 strains cultivated on CM agar plates for 4 days at 37 °C. b GFP::Snc1 strains cultivated on MM agar for 12–16 h at 28 °C and analysed via fluorescence microscopy. Secretory vesicles accumulate at the hyphal apex. Note that hyphae of T. thermophilus do not autofluoresce under the experimental condition used (see “Methods”, data not shown)

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