Fig. 3From: Deletion of the small GTPase rac1 in Trichoderma reesei provokes hyperbranching and impacts growth and cellulase productionGrowth, protein secretion and cellulase activity measurements of T. reesei K1 and Δrac1 liquid cultivations on different carbon sources. Five independently generated ∆rac1 strains and three biological replicates of the K1 strain were used for all liquid culture experiments. a Biomass formation on d-glucose, lactose and cellulose. Since cellulose is an insoluble carbon source, biomass formation is represented by the amount of internal protein. b Cellulase activity per biomass. Activity in the supernatants was determined by Azo-CMC liquid assay and was related to the amount of biomass. c Total protein in lactose supernatants determined by a Bradford assay. d Specific activity of lactose supernatant, the cellulase activity was plotted against the amount of total protein in the supernatantBack to article page