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Table 4 Gene knockout efficiency per target in Aspergillus niger

From: Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Gene target

Knockout efficiency (%)a

∆kusA protoplast survival (%) knockout repair DNA fragment (with/without)

brnA

100% (9/9)

0% (17/0)

crhA

100% (5/5)

6.9% (72/5)

crhB

100% (5/5)

0% (12/0)

crhC

83% (5/6)

82% (56/46)

crhD

66.7% (2/3)

0% (6/0)

crhE

80% (4/5)

87.5% (280/245)

crhF

100% (2/2)

0% (2/0)

crhG

100% (4/4)

0% (18/0)

  1. aKnockout efficiency among transformants checked by diagnostic PCR