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Table 3 A. niger colony expression levels of crh genes in different zones grown on xylose

From: Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

 

crhA

crhB

crhC

crhD

crhE

crhF

crhG

N402 zone 1

3.49

6.18

19.90

7.12

5.20

4.99

0.85

N402 zone 3

3.18

8.50

19.23

7.46

4.59

7.03

0.93

N402 zone 5

2.39

39.65

8.63

10.84

2.11

11.50

1.25

  1. Seven-day old sandwiched colonies grown on xylose were used for RNA isolation and subsequent microarray analysis [29]. Distinct zones of the mycelium (zone 1, 3, and 5) harvested form the colony. Zone 1 represents the oldest or central part of the colony. Zone 5 represents the youngest or peripheral part of the colony, whereas zone 3 represents the intermediate zone. Expression levels are represented as percentage of actin expression