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Table 1 All plasmids used in this study

From: Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Plasmid name (in text)

Technical name

Parental plasmid

Gene

An# (gene)

Gene name

Target sequence

Reference

pFC332

pFC332

–

–

–

–

–

[32]

pFC332_brnA-sgRNA

pTLL40.9

pFC332

NRRL3_01040

An14g05370

brnA

GGAGTGGTACCAATATGTGC

This study

pFC332_crhA-sgRNA

pTLL48.1

pFC332

NRRL3_10021

An11g01540

crhA

GGAGCTACCCATAATGATCC

This study

pFC332_crhB-sgRNA

pTLL58.1

pFC332

NRRL3_04809

An07g07530

crhB

GTAGGTCTTGCTCTCACACA

This study

pFC332_crhC-sgRNA

∆crhC-plasmid

pFC332

NRRL3_04315

An07g01160

crhC

GCTGTCGGTGCTGCAAGTCG

This study

pFC332_crhD-sgRNA

pTLL51.2

pFC332

NRRL3_02532

An01g11010

crhD

GACTGCTGTTGCGTTGGCTG

This study

pFC332_crhE-sgRNA

pTLL52.1

pFC332

NRRL3_01365

An13g02510

crhE

GCTCGTCTTGGCGTGATAGA

This study

pFC332_crhF-sgRNA

pTLL53.3

pFC332

NRRL3_07085

An16g02850

crhF

GTAACGACACATCTTTCGAC

This study

pFC332_crhG-sgRNA

pTLL60.1

pFC332

NRRL3_03998

An15g05350

crhG

GGTGTTGAGGGGGTTGCAAT

This study