Fig. 1From: Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungiDiagnostic PCR of crhA-G in the A. niger TLF39 and wild type (MA234.1) strains. ORFs removed for each crh gene in TLF39 show a downward band shift compared to MA234.1. a Exact band sizes are listed. b gDNA of TLF39 and MA234.1 was amplified with primer pairs for each crh gene (listed in Additional file 4: Table S1); PCR samples were loaded on 1% agarose gelsBack to article page