Fig. 1
From: Increasing access to microfluidics for studying fungi and other branched biological structures
An overview of the fabrication and assembly protocol for creating ready-to-use microfluidics. Standard photolithography is used to create microfluidic masters, conventional replicate molding with PDMS is used to produce microfluidic structures that are annealed to glass slides or coverslips. UV light exposure or autoclaving processes are used to sterilize the microfluidic platforms. Equilibration under vacuum is the conditioning step that immediately precedes vacuum packaging to produce a ready-to-use microfluidic platform