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Fig. 5 | Fungal Biology and Biotechnology

Fig. 5

From: Cpf1 enables fast and efficient genome editing in Aspergilli

Fig. 5

Cpf1-gRNA-mediated gene integration into a specific gene expression site. a Schematic overview of the experimental setup for marker-free specific Cpf1-gRNA-mediated gene insertion. b Plate layout to validate correct gene insertion in A. nidulans and A. niger. c, d visual inspection of A. nidulans and A. niger colonies, respectively, for development of color and production of red fluorescence. IS1 (Integration site 1, see text)

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