Substrate | PciGLOX1 | PciGLOX2 | PciGLOX3 |
---|---|---|---|
HMF | |||
Km (mM) | 15.66 ± 2.35 | 5.87 ± 2.04 | 6.35 ± 1.32 |
Kcat (s−1) | 1.59 ± 0.12 | 0.56 ± 0.09 | 0.75 ± 0.07 |
Kcat/Km (s−1 M−1) | 101.66 ± 0.01 | 96.04 ± 0.01 | 118.35 ± 0.01 |
DFF | |||
Km (mM) | 4.38 ± 0.1 | 0.21 ± 0.04 | 0.18 ± 0.05 |
Kcat (s−1) | 0.54 ± 0.24 | 4.80 ± 0.24 | 1.28 ± 0.09 |
Kcat/Km (s−1 M−1) | 124.39 ± 0.01 | 23,403.66 ± 0.01 | 7267.72 ± 0.01 |
FFCA | |||
Km (mM) | 0.85 ± 0.14 | 1.40 ± 0.39 | 0.61 ± 0.58 |
Kcat (s−1) | 0.03 ± 0.01 | 2.02 ± 0.03 | 0.04 ± 0.01 |
Kcat/Km (s−1 M−1) | 38.55 ± 0.01 | 1435.45 ± 0.01 | 72.03 ± 0.01 |