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Table 3 Kinetic parameters for the oxidation of HMF, DFF, and FFCA by the three PciGLOX isoenzymes. Standard deviations are presented as plus–minus values

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

Substrate

PciGLOX1

PciGLOX2

PciGLOX3

HMF

Km (mM)

15.66 ± 2.35

5.87 ± 2.04

6.35 ± 1.32

Kcat (s−1)

1.59 ± 0.12

0.56 ± 0.09

0.75 ± 0.07

Kcat/Km (s−1 M−1)

101.66 ± 0.01

96.04 ± 0.01

118.35 ± 0.01

DFF

Km (mM)

4.38 ± 0.1

0.21 ± 0.04

0.18 ± 0.05

Kcat (s−1)

0.54 ± 0.24

4.80 ± 0.24

1.28 ± 0.09

Kcat/Km (s−1 M−1)

124.39 ± 0.01

23,403.66 ± 0.01

7267.72 ± 0.01

FFCA

Km (mM)

0.85 ± 0.14

1.40 ± 0.39

0.61 ± 0.58

Kcat (s−1)

0.03 ± 0.01

2.02 ± 0.03

0.04 ± 0.01

Kcat/Km (s−1 M−1)

38.55 ± 0.01

1435.45 ± 0.01

72.03 ± 0.01