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Table 3 Kinetic parameters for the oxidation of HMF, DFF, and FFCA by the three PciGLOX isoenzymes. Standard deviations are presented as plus–minus values

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

Substrate PciGLOX1 PciGLOX2 PciGLOX3
HMF
Km (mM) 15.66 ± 2.35 5.87 ± 2.04 6.35 ± 1.32
Kcat (s−1) 1.59 ± 0.12 0.56 ± 0.09 0.75 ± 0.07
Kcat/Km (s−1 M−1) 101.66 ± 0.01 96.04 ± 0.01 118.35 ± 0.01
DFF
Km (mM) 4.38 ± 0.1 0.21 ± 0.04 0.18 ± 0.05
Kcat (s−1) 0.54 ± 0.24 4.80 ± 0.24 1.28 ± 0.09
Kcat/Km (s−1 M−1) 124.39 ± 0.01 23,403.66 ± 0.01 7267.72 ± 0.01
FFCA
Km (mM) 0.85 ± 0.14 1.40 ± 0.39 0.61 ± 0.58
Kcat (s−1) 0.03 ± 0.01 2.02 ± 0.03 0.04 ± 0.01
Kcat/Km (s−1 M−1) 38.55 ± 0.01 1435.45 ± 0.01 72.03 ± 0.01