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Table 2 Kinetic constants of PciGLOX3 on Methylglyoxal, glyoxal, glyoxylic acid and glycerol compared to PciGLOX1 and PciGLOX2

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

Substrate

PciGLOX1

PciGLOX2

PciGLOX3

Km (mM)

Kcat/Km (s−1 mM)

Km (mM)

Kcat/Km (s−1 mM)

Km (mM)

Kcat/Km (s−1 mM)

Methylglyoxal

1.3

58.4

0.2

7

0.1

75.8

Glyoxal

13.1

6.3

2.2

0.6

0.7

11.2

Glyoxylic acid

0.08

2136.3

0.1

17

0.5

15.3

Glycerol

660.5

0.04

9.4

0.06

5.5

1.4