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Table 2 Kinetic constants of PciGLOX3 on Methylglyoxal, glyoxal, glyoxylic acid and glycerol compared to PciGLOX1 and PciGLOX2

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

Substrate PciGLOX1 PciGLOX2 PciGLOX3
Km (mM) Kcat/Km (s−1 mM) Km (mM) Kcat/Km (s−1 mM) Km (mM) Kcat/Km (s−1 mM)
Methylglyoxal 1.3 58.4 0.2 7 0.1 75.8
Glyoxal 13.1 6.3 2.2 0.6 0.7 11.2
Glyoxylic acid 0.08 2136.3 0.1 17 0.5 15.3
Glycerol 660.5 0.04 9.4 0.06 5.5 1.4