Table 1 Substrate specificity of PciGLOX3 compared to previously characterized PciGLOX1 and PciGLOX2
Substrate (10 mM) | Activity (nkat/mg) | ||
|---|---|---|---|
PciGLOX1 | PciGLOX2 | PciGLOX3 | |
Methyl glyoxal | 814 | 291 | 90 |
Glyoxal | 392 | 163 | 68 |
Glyoxylic acid | 1562 | 384 | 59 |
3-Phenylpropionaldehyde | 10 | 135 | 51 |
Formaldehyde | 50 | 202 | 133 |
DL-glyceraldehyde | 11 | 66 | 26 |
Dihydroxyacetone | 108 | 157 | 64 |
Glycerol | 2 | 61 | 88 |
2,4-Dimethoxybenzaldehyde | ND | 2 | ND |
Veratraldehyde | ND | ND | ND |
4-Hydroxybenzaldehyde | ND | ND | ND |
Phenyl glyoxilic acid | ND | ND | ND |
Formic acid | ND | ND | ND |
d-glucose | ND | ND | ND |
d-galactose | ND | ND | ND |
d-xylose | ND | ND | ND |
Methanol | ND | ND | ND |