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Table 1 Substrate specificity of PciGLOX3 compared to previously characterized PciGLOX1 and PciGLOX2

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

Substrate (10 mM) Activity (nkat/mg)
PciGLOX1 PciGLOX2 PciGLOX3
Methyl glyoxal 814 291 90
Glyoxal 392 163 68
Glyoxylic acid 1562 384 59
3-Phenylpropionaldehyde 10 135 51
Formaldehyde 50 202 133
DL-glyceraldehyde 11 66 26
Dihydroxyacetone 108 157 64
Glycerol 2 61 88
2,4-Dimethoxybenzaldehyde ND 2 ND
Veratraldehyde ND ND ND
4-Hydroxybenzaldehyde ND ND ND
Phenyl glyoxilic acid ND ND ND
Formic acid ND ND ND
d-glucose ND ND ND
d-galactose ND ND ND
d-xylose ND ND ND
Methanol ND ND ND
  1. ND, activity not detected under these assay conditions