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Table 1 Colony forming units (CFU) and deletion efficiencies of the gaaX gene using a liquid handling robot to carry out the transformation

From: Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes

RNP (nM)

CFU

Colonies screened

Correct ΔgaaX

162

25

5

4

90

28

3

2

54

15

2

1

18

10

4

2

9

13

6

2

0

6

5

1