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Table 3 Transcriptome data of genes involved in IA biosynthesis and bioconversion

From: Itaconic acid degradation in Aspergillus niger: the role of unexpected bioconversion pathways

Locus tag Enzyme Old locus tag TargetP AB 1.13 WT AB 1.13 CAD AB1.13 #49B CitB#99
RPKM RPKM RPKM RPKM
  IA biosynthesis cluster
  Major facilitator superfamily transporter (mfsA)   PM 0.30 2.36 222.56 721.88
  Cis-aconitate decarboxylase (cadA)   Cytosol 3.54 6887.96 3116.06 7417.64
  Mitochondrial tricarboxylite transporter (mttA)   Mito 0.19 0.54 477.02 1257.78
  Citrate synthase
ANI_1_876084 Citrate synthase (citA) An09g06680 Mito 284.82 269.02 255.71 238.09
ANI_1_1226134 Citrate synthase (mcsA) An15g01920 Mito 76.60 61.89 82.55 85.78
ANI_1_1474074 Citrate synthase (citB) An08g10920 Cytosol 3.05 3.10 2.87 10,838.65
ANI_1_2950014 Citrate synthase (citC) An01g09940 Cytosol 463.10 370.88 438.08 96.44
  PrpD family (CadA like)
ANI_1_1536084 Immune-responsive protein An09g06220 Cytosol 0.30 0.23 0.36 0.43
ANI_1_2952014 Immune-responsive protein An01g09950 Cytosol 552.06 456.98 383.21 165.41
ANI_1_2948014 2-Methylcitrate dehydratase An01g09930 Cytosol 308.68 261.08 288.92 145.66
ANI_1_3352024 MmgE_PrpD superfamily protein An02g14730 Cytosol 5.97 7.29 5.81 10.08
  OahA class family
ANI_1_92174 Oxaloacetate acetylhydrolase (oahA) An10g00820 Cytosol 1803.48 1122.69 323.44 9.81
ANI_1_2054064 Oxaloacetate acetylhydrolase An07g08390 Cytosol 10.41 7.31 7.55 11.70
ANI_1_1800134 Oxaloacetate hydrolase class protein An15g07720 Cytosol 49.63 70.43 95.09 67.85
  IA bioconversion
ANI_1_1432064 Itaconyl-CoA transferase A (ictA) An07g00760 Mito 13.07 108.35 170.34 375.89
ANI_1_676164 CoA transferase superfamily enzyme An18g05120 Mito 11.10 9.36 11.79 9.99
ANI_1_2118064 Itaconyl-CoA hydratase A (ichA) An07g09220 Mito 7.32 60.28 146.61 232.65
ANI_1_316154 HTD2 protein An17g02190 Mito 23.69 22.26 22.39 26.75
ANI_1_1156014 Citramalate-CoA lyase (cclA) An01g08610 Mito 10.17 12.59 21.43 19.54
ANI_1_864144 Trans-aconitate 2-methyltransferase (tmtA) An16g06510 Mito 12.71 20.51 67.34 218.46
ANI_1_830134 UMTA methyltransferase family protein An15g06160 Per/Cytosol 15.52 11.45 52.11 23.02