Table 3 Transcriptome data of genes involved in IA biosynthesis and bioconversion
From: Itaconic acid degradation in Aspergillus niger: the role of unexpected bioconversion pathways
Locus tag | Enzyme | Old locus tag | TargetP | AB 1.13 WT | AB 1.13 CAD | AB1.13 #49B | CitB#99 |
|---|---|---|---|---|---|---|---|
RPKM | RPKM | RPKM | RPKM | ||||
| Â | IA biosynthesis cluster | ||||||
| Â | Major facilitator superfamily transporter (mfsA) | Â | PM | 0.30 | 2.36 | 222.56 | 721.88 |
| Â | Cis-aconitate decarboxylase (cadA) | Â | Cytosol | 3.54 | 6887.96 | 3116.06 | 7417.64 |
| Â | Mitochondrial tricarboxylite transporter (mttA) | Â | Mito | 0.19 | 0.54 | 477.02 | 1257.78 |
| Â | Citrate synthase | ||||||
ANI_1_876084 | Citrate synthase (citA) | An09g06680 | Mito | 284.82 | 269.02 | 255.71 | 238.09 |
ANI_1_1226134 | Citrate synthase (mcsA) | An15g01920 | Mito | 76.60 | 61.89 | 82.55 | 85.78 |
ANI_1_1474074 | Citrate synthase (citB) | An08g10920 | Cytosol | 3.05 | 3.10 | 2.87 | 10,838.65 |
ANI_1_2950014 | Citrate synthase (citC) | An01g09940 | Cytosol | 463.10 | 370.88 | 438.08 | 96.44 |
| Â | PrpD family (CadA like) | ||||||
ANI_1_1536084 | Immune-responsive protein | An09g06220 | Cytosol | 0.30 | 0.23 | 0.36 | 0.43 |
ANI_1_2952014 | Immune-responsive protein | An01g09950 | Cytosol | 552.06 | 456.98 | 383.21 | 165.41 |
ANI_1_2948014 | 2-Methylcitrate dehydratase | An01g09930 | Cytosol | 308.68 | 261.08 | 288.92 | 145.66 |
ANI_1_3352024 | MmgE_PrpD superfamily protein | An02g14730 | Cytosol | 5.97 | 7.29 | 5.81 | 10.08 |
| Â | OahA class family | ||||||
ANI_1_92174 | Oxaloacetate acetylhydrolase (oahA) | An10g00820 | Cytosol | 1803.48 | 1122.69 | 323.44 | 9.81 |
ANI_1_2054064 | Oxaloacetate acetylhydrolase | An07g08390 | Cytosol | 10.41 | 7.31 | 7.55 | 11.70 |
ANI_1_1800134 | Oxaloacetate hydrolase class protein | An15g07720 | Cytosol | 49.63 | 70.43 | 95.09 | 67.85 |
| Â | IA bioconversion | ||||||
ANI_1_1432064 | Itaconyl-CoA transferase A (ictA) | An07g00760 | Mito | 13.07 | 108.35 | 170.34 | 375.89 |
ANI_1_676164 | CoA transferase superfamily enzyme | An18g05120 | Mito | 11.10 | 9.36 | 11.79 | 9.99 |
ANI_1_2118064 | Itaconyl-CoA hydratase A (ichA) | An07g09220 | Mito | 7.32 | 60.28 | 146.61 | 232.65 |
ANI_1_316154 | HTD2 protein | An17g02190 | Mito | 23.69 | 22.26 | 22.39 | 26.75 |
ANI_1_1156014 | Citramalate-CoA lyase (cclA) | An01g08610 | Mito | 10.17 | 12.59 | 21.43 | 19.54 |
ANI_1_864144 | Trans-aconitate 2-methyltransferase (tmtA) | An16g06510 | Mito | 12.71 | 20.51 | 67.34 | 218.46 |
ANI_1_830134 | UMTA methyltransferase family protein | An15g06160 | Per/Cytosol | 15.52 | 11.45 | 52.11 | 23.02 |