Table 1 Primers used for strain construction in this study
Primer name | Sequence 5′–3′ | References |
|---|---|---|
5′cre1_NotI fwd | GCGGCCGCTGGAGGTGACGAGAAGAAAAATTCAGG | This study |
5′cre1_XmaI rev | CCCGGGAGTCAAAAAGCAAGTACGCGACGTTG | This study |
hph_XmaI_BamHI | CCCGGGTTGGATCCAGGGAGACGAGGTTGTGATGAATAC | This study |
hph_SpeI rev | ACTAGTAAGTAGCACCGCTGTCGTCTG | This study |
5Pcre1_NotI fwd | GCGGCCGCAGCCAAGACTCAGCATAAAGAGGTTG | This study |
5Pcre1_XmaI rev | CCCGGGAGGTACCAAACAAAGCGAGCAAGTAC | This study |
ptef_BspEI fwd | TCCGGATGTGTGACAGCTCGCGCAG | This study |
ptef_NdeI rev | CATATGTGACGGTTTGTGTGATGTAGCGTG | This study |
cre1-96_NdeI fwd | CATATGATGCAACGAGCACAGTCTGCC | This study |
Cre1-96_BamHI rev | GGATCCTTAGAAAAAAAAGCAGGTAATGGAGGTGC | This study |
cre1-96_BspEI fwd | TCCGGAATGCAACGAGCACAGTCTGCC | This study |
cre1-96-TAA_NdeI rev | CATATGGAAAAAAAAGCAGGTAATGGAGGTGC | This study |
linker_NheI rev | GCTAGCGCGGGGGGCGCAC | This study |
linker_NdeI fwd | CATATGCACAACATGGTCAAGCAGAAGC | This study |
YFP_NheI fwd | GCTAGCATGGTCAGCAAGGGCGAGG | This study |
YFP_BamHI rev | GGATCCCTTGTACAGCTCGTCCATGCCG | This study |