Fig. 9

Cellulase activity in presence of a constitutively expressed Cre1-96. a T. reesei strains Rut-C30 (blue squares), Rut-C30OEcre1-96 (purple squares) and Rut-C30Δcre1-96 (orange squares) were cultivated in liquid medium supplemented with 1% (w/v) lactose for 36, 48 and 60 h. The endo-cellulolytic activities in the culture supernatants were measured in biological and technical duplicates and normalized to the biomass. The enzymatic activities are given as means and the error bars indicate the standard deviations. Error bars are not shown for standard deviations ≤ 3.5%. The values were statistically analysed by an ordinary one-way ANOVA and a Tukey’s posthoc test in a confidence interval of 95%, and asterisks indicate significant differences. b For the growth assays, the T. reesei strains Rut-C30, Rut-C30OEcre1-96 and Rut-C30Δcre1-96 were pre-grown on MEX plates and were then transferred to MA medium plates supplemented with 1% (w/v) CMC or 1% (w/v) lactose. Plates were incubated at 30 °C and pictures were taken after 24, 48, 60 and 84 h