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Fig. 8 | Fungal Biology and Biotechnology

Fig. 8

From: Truncation of the transcriptional repressor protein Cre1 in Trichoderma reesei Rut-C30 turns it into an activator

Fig. 8

Chromatin accessibility under cellulase inducing conditions. T. reesei strains Rut-C30 (blue symbols) and Rut-C30Δcre1-96 (orange symbols) were cultivated in liquid medium supplemented with 1% (w/v) lactose for 36 (squares), 48 (triangles) and 60 h (dots). CHART-PCR and transcript analysis of cbh1 (b), xyn1 (c) and xyr1 (a) were performed in biological and technical replicates. Both sets of data were normalized to the housekeeping genes sar1 and act. The relative transcript ratios are given as means and plotted on the x-axis and the chromatin accessibility indices (CAI) are plotted on the y-axis. Error bars indicate the standard deviations. All CAI values were statistically analysed in a confidence interval of 95% and asterisks indicate significant differences amongst the chromatin-related data

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