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Fig. 7 | Fungal Biology and Biotechnology

Fig. 7

From: Truncation of the transcriptional repressor protein Cre1 in Trichoderma reesei Rut-C30 turns it into an activator

Fig. 7

Chromatin immunoprecipitation of Cre1-96::eYFP. The T. reesei strain QM6acre1-96::eyfp was cultivated for 16 h in 20 mL MEX medium supplemented with 1% (w/v) d-glucose. After crosslinking, the chromatin was enzymatically fragmented by MNase treatment and Cre1-96::eYFP targeted DNA was enriched using anti-GFP antibodies. The relative amount of DNA was measured by qPCR. The immunoprecipitated DNA was normalized to the input control. The resulting ratio for the xyr1 gene (grey bar) was further normalized to the ratio of housekeeping gene sar1, which is set to 1 (black bar). The values were statistically analysed in a confidence interval of 95% and the asterisk indicates a significant difference

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