Fig. 6

Confocal microscopy analysis of the localization of Cre1-96. The T. reesei strain QM6acre1-96::eyfp was cultivated in liquid medium supplemented with 1% (w/v) d-glucose (a) or 1% (w/v) lactose (b). For the visualization of fungal nuclei a Hoechst staining was performed. The following pictures were imaged to localize Cre1-96::eYFP in the fungal hyphae: detection of eYFP (EYFP), detection of the nuclei within the fungal cells (Hoechst), overlay of eYFP and Hoechst emissions (Merge), and brightfield image (DIC). Scales are given in the DIC pictures