Fig. 4

Transcript levels of xyl1, bga1 and Tre3405 in absence and presence of Cre1-96. T. reesei strains Rut-C30 (blue bars) and both Rut-C30Δcre1-96 strains (yellow and orange bars) were cultivated in liquid medium supplemented with 1% (w/v) lactose for 36, 48 and 60 h. Transcript analyses of xyl1 (a), bga1 (b) and Tre3405 (c) were performed in biological and technical duplicates by qPCR, data were normalized to the housekeeping genes sar1 and act, and referred to the respective transcript levels of Rut-C30 at 36 h. The relative transcript ratios are given as means and the error bars indicate the standard deviations. All values were statistically analysed in a confidence interval of 95% and asterisks indicate significant differences