Fig. 2

Cellulolytic and xylanolytic activities in absence and presence of Cre1-96. T. reesei strains Rut-C30 (blue squares) and both Rut-C30Δcre1-96 strains (yellow and orange squares) were cultivated in liquid medium supplemented with 1% (w/v) lactose or d-glucose for 36, 48 and 60 h. The endo-cellulolytic on lactose (b) and on glucose (d) as well as the xylanolytic activities on lactose (c) in the culture supernatants were measured in biological and technical duplicates and normalized to the biomass measured as wet weight (a). The enzymatic activities are given as means and the error bars indicate the standard deviations. The values were statistically analysed by an unpaired two-tailed t test in a confidence interval of 95%, and asterisks indicate significant differences. e Relative cre1-96 transcript ratios were analysed for both deletion strains and Rut-C30 grown on lactose. Transcript analysis was performed in biological and technical duplicates by qPCR, data were normalized to the housekeeping genes sar1 and act, and referred to the transcript level of Rut-C30 at 36 h. The relative transcript ratios are given as means and the error bars indicate the standard deviations. Error bars are not shown for standard deviations ≤ 3.5%. All values were statistically analysed in a confidence interval of 95%; ‘n.d.’ means not detected